中国鹿亚科动物mtDNA控制区序列差异及其遗传分化

Sequence Difference of Mitochondrial DNA Control Region and Genetic Differentiation of Cervinae in China

[目的]探讨中国鹿亚科动物的遗传分化。[方法]通过PCR直接测序的方法获得我国鹿亚科物种线粒体DNA控制区(D-loop)全序列,结合GenBank检索到的鹿亚科其他动物同源序列,用生物软件进行统计分析。[结果]我国鹿亚科动物D-loop区序列全长在921～1 072 bp,碱基T、A、C、G的平均含量分别为32.1%、30.2%、22.7%及15.0%,各物种间的遗传距离范围在0.062～0.106之间,处于属间差异。系统进化树结果表明梅花鹿、马鹿和白唇鹿亲缘关系较近,它们与水鹿构成一组进化枝,坡鹿与麋鹿构成一组进化枝,豚鹿单独构成一个进化枝。[结论]该研究支持麋鹿和豚鹿并入鹿属的观点,我国的鹿亚科动物的分化时间约为155～260万年。

[Objective] The study aimed at analyzing Genetic Differentiation of Cervinae.[Method] The complete mitochondrial DNA control region of 5 species of Cervinae was determined by direct DNA sequencing,Combined with homologous sequences of others Cervinae downloaded from GenBank,then analyzed through biology sofeware.[Conclusion] The lengths of their control region were 921-1 072 bp,the nucleotide content of T,A,C,and G was 32.1%、30.2%、22.7% and 15.0% separately,genetic distance of each species ranged from 0.062-0.106,they belonged to level of inter-genus.the molecular phylogenetic tree indicated that red deer,skia deer and White-lipped Deer had close relationship,they constituted one branch with sambar deer,Eld’s d eer and elk deer constituted the second branch,and hog deer constituted the third branch alone.[Result] Hog deer and fallow deer should be incorporated in cervus,divergence time were about 1.55-2.60 million years among Cervinae in China.