番茄幼苗根系总蛋白SDS-PAGE方法的优化

Optimization of SDS-PAGE Procedures for Analyzing the Total Protein in Seedling Roots of Lycopersicon esculentum

[目的]建立一套适合番茄幼苗根系蛋白质组分析的SDS-PAGE方法和获得清晰的电泳图谱。[方法]以番茄幼苗根系为试材,对根系蛋白的提取方法和上样量等因素进行了优化。[结果]上样量为60μg时,改良后的TCA-丙酮沉淀法提取的蛋白能够获得分辨率较高、图像清晰、重复性好的SDS-PAGE图谱。[结论]该法可以基本满足番茄幼苗根系蛋白质组学的研究。

[Objective] This study aimed to develop an efficient SDS-PAGE method suitable for analyzing the total protein of Lycopersicon esculentum seedling roots.[Method] By using the L.esculentum seedling roots as the experimental material,the protein extraction,sample volume and other factors influencing the final electrophoresis pattern were respectively optimized.[Result] The high-resolution,clear and reproducible SDS-PAGE pattern could be obtained when the protein was extracted with modified TCA-acetone precipitation and the sample volume for electrophoresis was 60 μg.[Conclusion] This optimized method could basically meet the requirements for study on the proteome of tomato seedling roots.