川产道地药材三叶黄连HPLC指纹图谱研究

Study on the HPLC Fingerprint of RHIZOMA COPTIDIS in Sichuan

[目的]建立川产道地药材三叶黄连的指纹图谱检测标准。[方法]以盐酸小檗碱为参照物,采用依利特SinoChrom ODS-BP(250mm×4.6 mm,5μm)色谱柱,以乙腈-浓度0.1%乙酸和10 mmol乙酸铵溶液为流动相梯度洗脱,流速为1.0 ml/min,检测波长为345nm。[结果]药材有14个共有峰,共有峰的参数符合"中药注射剂指纹图谱技术要求"的有关规定。[结论]该方法准确、重复性好,建立的指纹图谱检测标准,可作为川产道地药材三叶黄连质量评价与品种鉴别的重要依据之一。

[ Objective ] To establish the fingerprint detecting standard for RHIZOMA COPTIDIS in Sichuan. [ Method ] Using berberine hydro- chloride as the reference substance, the separation was carried out on a SinoChrom ODS -BP （250 mm x 4.6 ram, 51~m） with the detection wavelength at 345 nm. Following the injection of sample solution, a gradient elution with methanol -0. 1% caproic acid and 10 mmol/L ammoni- um acetate as mobile phase was initiated at a flow rate of 1.0 ml/min. [ Result] A total of 14 common peaks were found in the chromatogram of RHIZOMA COPTIDIS, the parameter of which met with the stipulation of Chinese Medicine Injection Fingerprint Technical Requirements. [ Conclu- sion] The method is simple and accurate with a good reproducibility and can be used in quality evaluation and variety discrimination of RHIZO- MA COPTIDI~ in ~i^hllnn.