湖北麦冬叶片快繁体系的建立

Establishment of Rapid Propagation System from the Leaves of Hubei Liriope spicata var.prolifera Y.T.Ma

[目的]建立湖北麦冬叶片的快繁体系。[方法]以湖北麦冬幼嫩叶片为外殖体,以MS为基本培养基,向其中添加不同浓度的6-BA、NAA、GA后进行培养,探讨外源激素对其愈伤形成、不定芽分化、增殖与不定根形成的影响。[结果]采用MS+0.1～2.0 mg/L 6-BA+0.01～0.5 mg/L NAA培养基,愈伤诱导率在20%以上;采用MS+0.1～1.0 mg/L 6-BA+0.01～0.5 mg/LNAA+0.1～1.0 mg/L GA培养基,对芽的生长及增殖效果好,说明GA具有提高成苗率的作用;采用1/2MS+0.1～0.5 mg/L NAA或0.1 mg/L IBA或0.1～0.5mg/L IAA,20 d内生根率可达97%以上。在腐叶土∶珍珠岩=5∶3的基质中驯苗,成活率可达90%以上。[结论]优选出了湖北麦冬叶片的最佳快繁体系,为麦冬的开发利用提供了理论依据。

[Objective] To establish the rapid propagation system from the leaves of Hubei Liriope spicata var.prolifera Y.T.Ma.[Method] The effects of external hormones on the callus formation,adventitious bud differentiation and proliferation,adventitious root formation were explored after the culture of Hubei L.spicata tender leaves as explant in MS minimal medium with the addition of 6-BA,NAA and GA.[Result] The callus induction rate of L.spicata leaves was more than 20% in the medium of MS＋0.1-2.0 mg/L 6-BA＋0.01-0.5 mg/L NAA.In the medium of MS＋0.1-1.0 mg/L 6-BA＋0.01-0.5 mg/LNAA＋0.1-1.0 mg/L GA,the growth and proliferation effects of buds were good,which pointed out that GA could improve the surviving rate of seedlings.The medium of 1/2MS＋0.1-0.5 mg/L NAA or 0.1 mg/L IBA or 0.1-0.5 mg/L IAA could make the rooting rate reach 97% during 20 days.If the sprouts were transplanted into the substrate in which the rate between leaf mold and perlite was 5∶3,the survival rate could reach over 90%.[Conclusion] The optimum rapid propagation system from leaves was selected,which provided theoretical basis for the development and utilization of L.spicata.