厚朴叶多糖铁复合物中铁的含量测定

Determination of Fe(Ⅲ) from the Polysaccharide-Fe(Ⅲ) Complex of CORTEX MAGNOLIAE OFFICINALIS Leaves

[目的]测定厚朴叶多糖铁复合物中铁的含量。[方法]从厚朴叶中提取多糖,在碱性条件下,厚朴叶多糖与氧化铁反应合成厚朴叶多糖铁(Ⅲ)复合物(LPC)。其中的铁(Ⅲ)含量用邻菲啰啉紫外分光光度法进行测定。[结果]在铁(Ⅲ)浓度为0～0.196 7 mg/ml时,浓度与吸光度呈现良好的线性关系(r=0.999 8);平均回收率为98.83%,RSD为1.60%;厚朴叶多糖铁(Ⅲ)复合物中铁(Ⅲ)含量为31.42%。[结论]该方法简便迅捷、可靠,可用于厚朴叶多糖铁复合物中铁的含量测定。

[Objective] To determine the Fe（Ⅲ） content in polysaccharide-Fe（Ⅲ） complex of CORTEX MAGNOLIAE OFFICINALIS leaves.[Method] Polysaccharide was extracted from the leaves of CORTEX MAGNOLIAE OFFICINALIS.Under alkaline condition,polysaccharide-Fe（Ⅲ） complex was formed.And the content of Fe（Ⅲ） was determined by UV spectrophotometry.[Result] when the Fe（Ⅲ） content was between 0 and 0.196 7 mg/ml,Absorbance and concentration showed good linear relationship（r=0.999 8）;the average recovery rate was 98.83% and RSD was 1.60%;Fe（Ⅲ） content in polysaccharide-Fe（Ⅲ） complex of CORTEX MAGNOLIAE OFFICINALIS leaves was 31.42%.[Conclusion] This method was rapid and reliable and could be used for the Fe（Ⅲ） content in polysaccharide-Fe（Ⅲ） complex of CORTEX MAGNOLIAE OFFICINALIS leaves.