双碱配合提取啤酒厂剩余污泥蛋白质的研究

Research on Extracting Protein with Dual-alkali from Brewery Excess Sludge

[目的]提高啤酒厂剩余污泥中蛋白质的提取率。[方法]分别选取水解温度、水解时间、NaOH或Ca(OH)2投加率为影响因素,以蛋白质提取率为评价指标,进行正交试验,并以此为依据采用双碱法优化污泥水解的工艺条件。[结果]双碱配合水解污泥提取蛋白质的最佳条件为NaOH投加率(占干污泥的质量分数)8%,Ca(OH)2投加率15%,水解温度80℃,水解时间7 h,此时反应体系pH为13。在最优条件下,蛋白质提取率可达60.03%。[结论]该研究可为啤酒厂污泥的资源化利用提供科学依据。

[Objective] The paper aimed to improve the extraction rate of protein from brewery excess sludge.[Method] Hydrolysis temperature,hydrolysis time,NaOH or Ca（OH）2 dosing ratio were selected as influence factors respectively,and the extraction rate of protein as evaluation value for the orthogonal experiment.Dual-alkali hydrolyzing method was used to optimize the sludge hydrolysis technology conditions based on these factors.[Result] The optimum conditions were as follows：NaOH dosing ratio（ratio of NaOH to the total dry sludge mass） 8%,Ca（OH）2 dosing ratio 15%,hydrolysis temperature 80 ℃,hydrolysis time 7 h.Under these conditions,pH value of the reaction system was 13 and extraction rate of protein could reach 60.03%.[Conclusion] The research provided a scientific basis for resource utilization of brewery excess sludge.