土茯苓对肾性高血压大鼠血压的调节作用和机制

Regulatory Effects and Mechanism of Smilax glabra on Blood Pressure in Renovascular Hypertensive Rats

目的观察土茯苓对肾性高血压大鼠血压及血液内血管活性物质的调节作用。方法用两肾两夹法造大鼠高血压模型,将造模成功的大鼠按收缩压分层,分成模型对照组、高(土茯苓6 g/kg)、中(土茯苓3 g/kg)、低(土茯苓1.5 g/kg)剂量组和阳性组(卡托普利5 mg/kg);试验期间各组经口灌胃给予相应药物,每日1次,连续4周。每周测定血压1次,末次给药后,测定大鼠血液中ANP、NO、ET、CGRP和AngII水平。结果土茯苓显著降低了肾性高血压大鼠SBP、DBP和MBP(P<0.05,P<0.01),同时显著降低了ANP、ET的水平(P<0.05,P<0.01),升高了NO的水平(P<0.05)。结论土茯苓具有一定的降血压作用,其作用途径可能通过降低ANP、ET和升高NO的水平,而发挥血压调节的作用。

Objective To observe the regulatory effects of Smilax glabra on blood pressure and vasoactive substances in renovascular hypertensive rats. Method Seventy healthy adult male rats were used to establish the model of renovascular hypertension by two-kidney two-clip procedure. Model rats were divided into model control group, high （Smilax glabra 6 g/kg） , moderate （Smilax glabra 3 g/kg） , low （Smilax glabra 1.5 g/kg） dose groups and the positive control group （captopril 5 mg/kg） according to systolic blood pressure in the renal hypertensive rats. Rats of the drug groups were administrated the corresponding drugs by once daily for 4 weeks,and measured blood pressure once a week. After the last administration, the blood ANP, NO, ET, CGRP and Ang II of the rats were measured. Result Smilax glabra significantly reduced SBP, DBP and MBP in the renal hypertensive rats （P 〈 0. 05, P 〈 0.01 ） , significantly reduced the ANP, ET levels （ P〈 0.05, P 〈 0.01 ） , and increased the NO level （ P 〈 0.05 ）. Conclusion Smilax glabra has a blood pressure lowering effect and regulates the blood pressure by reduced ANP, ET, and increased NO levels.