幽门螺杆菌感染的冠心病患者血清IL-8、IL-6、TNF-α和NO水平

The Relationship of Helicobacter pylori Infection with Serum Levels of IL-8,IL-6,TNF-α and NO in Patients with Coronary Heart Disease

目的:探讨幽门螺杆菌(Hp)感染的冠心病患者血清炎症因子白介素-6(IL-6)、白介素-8(IL-8)、肿瘤坏死因子(TNF)和一氧化氮(NO)含量。方法:308例患者分为急性冠脉综合征(ACS)组和慢性心肌缺血综合征(CCS)组,120例健康志愿者为对照组,用ELISA法检测血清IL-6、IL-8水平,放射免疫法检测血清TNF-α水平,镀铜镉粒还原法间接测定血清NO浓度,14C尿素呼气实验检测HP。结果:ACS组及CCS组IL-6、IL-8、TNF-α明显高于对照组,NO明显低于对照组;ACS Hp(+)组及CCS Hp(+)组IL-6、IL-8、TNF-a高于Hp(-)组,NO明显低于Hp(-)组;Hp CagA(+)组IL-8、TNF-α明显高于Hp CagA(-)组及Hp(-)组。结论:IL-6、IL-8、TNF-α和NO均参与Hp感染的冠心病的病理过程。

Objective： To investigate the role of serum levels of interleukin-6（IL-6）,interleukin-8（IL-8）,tumor necrosis factor-α（TNF-α）and nitric oxide（NO）in development of coronary heart disease in Helicobacter pylori（Hp）infected patients with coronary heart disease,and to explore possible molecular mechanism of HP infection leading to coronary heart disease.Methods： A total of 308 patients were divided into acute coronary syndrome group（group ACS） and chronic myocardial ischemic syndrome group（group CCS）,and 120 healthy volunteers were selected as control group.Serum levels of IL-6,IL-8 and CagA-Hp IgG were detected with enzyme linked immunosorbent assay.Serum level of TNF-α was tested with radioimmunoassay.Serum NO was indirectly detected with chrome reduction method using plated copper.Hp infection was detected with 144C Urea breath test（144C-UBT）.Results： Serum levels of IL-8,IL-6 and TNF-α in group ACS and group CCS were significantly higher than those in group C,while serum NO levels in the two groups were significantly lower than that in group C.The levels of IL-8,IL-6 and TNF-α of HP（＋）patients in groups ACS and CCS were significantly higher than those of HP（-）patients,and NO level of HP（＋） patients was significantly lower than that of HP（-）patients.IL-8 and TNF-α levels of Hp CagA（＋） patients were significantly higher than those of Hp CagA（-）patients and Hp（-）patients.Conclusions： IL-8,IL-6,TNF-α and NO are involved in pathological progress of coronary heart disease（CHD）.Detection of IL-8,IL-6,TNF-α and NO levels and CagA classification of HP strains in HP（＋） patients with coronary heart disease are helpful to monitoring disease and predicting prognosis.