豌豆蚜唾液蛋白家族的克隆及在不同发育阶段的表达

Cloning and expression profiling of a saliva protein family at different developmental stages in Acyrthosiphon pisum(Hemiptera:Aphididae)

豌豆蚜Acyrthosiphon pisum是一种重要的刺吸式害虫,其分泌的唾液对取食寄主植物和传播植物病毒有重要的作用。为了探讨蚜虫唾液蛋白的功能,本研究克隆了在豌豆蚜唾液腺高表达的一个未知功能的蛋白家族,该家族包括13个基因,编码14种蛋白,其中4个基因在唾液腺高表达。这个家族是蚜虫特有的蛋白家族,富含半胱氨酸,有14个半胱氨酸高度保守,其中6个半胱氨酸形成3个保守的CXXC结构域。通过与基因组比对,发现这个家族的基因没有内含子,分布在基因组的9个scaffold上。用半定量逆转录PCR检测了每个成员在豌豆蚜不同发育阶段的表达,结果显示这个家族没有发育阶段特异性。推测这个家族的表达可能具有组织特异性,有氧化还原酶或DNA甲基化酶的功能。

The pea aphid,Acyrthosiphon pisum,is an important piercing-sucking pest.The secreted saliva plays roles in feeding host plants and virus transmission.In order to explore the function of saliva proteins,we cloned an unknown protein family that is highly expressed in salivary glands of the pea aphid.The family consists of 13 genes,which encode 14 proteins,and four of them are highly expressed in salivary glands.The family is unique to aphids and enriched in cysteine.Fourteen cysteines are conserved,6 of which form three CXXC domains.Genes of this family have no introns and are distributed in 9 scaffolds of the aphid genome.Semi-quantitative reverse transcription PCR showed that there was no stage-specific expression for this family.We speculate that the expression of this family may be tissue-specific and this protein family probably has the functions of oxidoreductases or DNA methyltransferases.