富油金藻Isochrysis sp．CCMM5001脂肪酸成分分析及其制备的生物柴油质量分析

Analysis of Fatty Acid Composition of Oil-rich Isochrysis sp. CCMM5001 and Quality of Prepared Bio-diesel

采用GC～MS法对富油金藻Isochrysissp．CCMMS001中的脂肪酸及其制备的生物柴油进行成分含量分析，并对所制生物柴油的密度、运动粘度、闪点、酸值及铜片腐蚀度等性能进行测定。结果显示，富油金藻中脂肪酸主要为C14，C16，C18和C22，总脂肪酸含量占粗脂的82．93％；所制生物柴油产品主要成分为C16和C18脂肪酸甲酯，二者占产品总量的93％；生物柴油的密度，运动粘度，闪点，铜片腐蚀度等基本符合市场上流通的生物柴油的要求标准。实验表明，具有较高脂肪酸含量的富油金藻作为制备生物柴油的原料具有重要的意义，且所制得的生物柴油可以作为当前市场使用柴油的一个理想替代品而进一步深入研究。

We used the method of GC-MS to analysis the composition and content of the fatty acid in oil-rich Isochrysis sp. CCMMS001 to prepare bio-diesel, and determined the characteristics of bio-diesel such as density,viscosity,flash point,acid value and degree of copper corrosion. The result showed that fatty acids in the oil-rich Isochrysis sp. CCMMS001 are mainly C16 ,C18 and C22, total fatty acid content is 82.93 % in crude lipid;the main content of the prepared bio-diesel is C16 and C18 fatty acid methyl esters, which is 93% of the total product. The density,viscosity ,flash point,acid value and degree of copper corrosion of the prepared bio-diesel basically conform to the standard of market circulation . The experiment showed that using oil-rich Isochrysis sp. CCMM5001 which has higher fatty acids content as a raw material for preparation of bio-diesel is significant, and needs further study. The prepared bio-diesel can be an ideal substitute for prevailing diesel in the market.