两种海洋细菌对海底沉积物微生物燃料电池产电效能及其阳极表面细菌群落的影响

The Influences of Two Marine Bacteria to the Bacterial Community Compositions of the Anode and the Electricity Production Efficacy of Marine Benthic Microbial Fuel Cell

从海底沉积物微生物燃料电池（Benthic microbial fuel cell，BMFC）模型的阳极表面分离得到了异养可培养优势细菌蜡样芽孢杆菌（Bacillus cereus）LYX03和一株海洋新菌一海洋底泥食冷菌（Algoriphagus faecimaris ）LYX05，并分别加入BMFC阳极表面；通过电压输出功率变化和变性梯度凝胶微生物指纹图谱技术结合16SrDNA序列分析，进一步研究其对BMFC产电效能和阳极表面菌群的影响。同时进一步探讨了葡萄糖和LB培养基对BMFC的产电效能和阳极表面菌群的影响。结果表明海洋底泥食冷菌和LB培养基的直接加入导致该电池模型的产电效能显著降低，而蜡样芽孢杆菌及其胞外DNA，葡萄糖的添加对输出电压无显著影响。海洋细菌和碳源的加入对电池阳极表面菌群的种类组成有较大的扰动，导致细菌种类多样性的降低，其中华丽杆菌（Chryseobacteriurn sp．）为易感种类。

The dominant strain Bacillus cereus LYX03, isolated from the heterotrophic culture of marine benthic microbial fuel cell （MBMFC）, and a newly identified marine bacteria Algoriphagus faecimaris LYX05 isolated from the same source, were injected to the surface of anode of MBMFC. The bacterial community compositions and the electricity production efficacy of MBMFC were analyzed by means of bacterial fingerprinting technique denaturing gradient gel electrophoresis, 16S rDNA sequencing and voltam meter analyses. The effects of glucose and LB medium to the bacterial community compositions and electricity production efficacy were further analyzed. The results indicated that Algoriphagus faecimaris LYX05 and LB medium decreased the electricity production efficacy of MBMFC significantly. While the injections of Bacillus cereus LYX03, extracellular DNA and glucose did not generated significant effects. The injections of two marine bacterial strains and carbon source such as glucose, and LB medium showed a large disturbance of microbial communities of the anodes with decreased bacterial diversity. Chryseobacterium sp. was the most vulnerable species.