摘要
从南极土样中分离得到一株能大量分泌紫红色色素的地丝霉属(Geomyces)的真菌。通过对培养基和温度的选择,确定了该菌株产色素的基础培养基,发现较低的温度有利于色素的积累。另外,对7种树脂吸附量、解吸率和提取效率进行了比较,选择了HPD-722型树脂吸附色素,洗脱剂为70%乙醇。经过大孔树脂吸附法提取,色素色价从12.68提高到144.05,增高10.4倍,效果非常明显。碳源的加入对电池阳极表面菌群的种类组成有较大的扰动,导致细菌种类多样性的降低,其中华丽杆菌(Chryseobacterium sp.)为易感种类。
A Geomyces species was isolated from Antarctica soil, which produces plenty of red pigment. With screening test of culture medium and temperature, the basic medium was determined in which the pigment was produced, and it was discovered that low temperature benefited the accumulation of pigment in the fungus. In addition, compared the adsorption capacities and desorption rates of several macroporous absorption resin, HPD-722 resin and 70% ethanol were determined for pigment absorption and eluting reagent. After extraction and absorption by macroporous resin, the Color Value Units E1cm^1% increased from 12.68 to 144.05, a 10.4 times increase was recorded.
出处
《海洋科学进展》
CAS
CSCD
北大核心
2011年第A01期116-121,共6页
Advances in Marine Science
基金
海洋公益性行业科研专项--盐生植物生产纤维素乙醇与电厂废气联产生物柴油研究与示范(201005031),海洋生物活性物质国家标准样品研制与研发技术体系构建(200905022-2),低温纤维素酶的开发研究(201005020)
“十二五”国家科技计划课题--微藻减排燃煤烟气及二氧化碳和炼制生物柴油的关键技术和产业化示范
关键词
南极
地丝霉属
天然色素
大孔吸附树脂
Antarctica
Geomyces
natural pigment
macroporous absorption resin