摘要
DNA条形码技术，系使用短的标准DNA片段对物种进行快速、准确的识别和鉴定的方法，该技术已成为现代生物分类方法的研究热点。以在山东省威海市双岛湾和楮岛附近海域采集的海草（HCSD1，HCSD2，HCSD3）为研究对象，分别采用18SrDNA，ITS和matK基因序列作为DNA条形码对其进行系统分类。结果表明，18SrDNA基因序列保守性最强，ITS和matK基因序列的多态性高于18SrDNA基因序列；而基于matK基因序列的分类效果与形态鉴定最相符。所采集的海草样品HCSDl和HCSD2为大叶藻（Zostera．marina），HCSD3为丛生大叶藻（Zostera．caespitosa）。这表明matK基因序列适用于我国北方海草的种类识别鉴定。
DNA Barcoding is a taxonomic technique that uses short genetic DNA sequence（s） for fast species identification and it has been among hot topics in modern taxonomy methods. In this study, the potential barcode sequences of 18S rDNA, ITS and matK genes of seagrass samples（HCSD1、HCSD2、HCSD3） from Shuangdao Bay and Chudao of Weihai, Shandong, were cloned for phylogeny analysis. The results showed that the 18S rDNA sequence was highly conserved. ITS and matK genes showed a higher degree of polymorphism than 18S rDNA sequence and DNA taxonomy of matK sequence was consistent with morphological taxonomy. Samples HCSD1 and HCSD2 were identified as Z. marina, and samples HCSD3 was identified as Z. caespitosa. The results indicated that matK sequence is a good DNA barcode for seagrass delimitation in North Coastal of China.
出处
《海洋科学进展》
CAS
CSCD
北大核心
2011年第A01期136-143,共8页
Advances in Marine Science
基金
国家科技支撑计划项目--重要海湾海岸带典型受损生境修复关键技术研究与示范（2010BAC68B01）
