摘要
利用EMS诱变筛选手段分离到一株拟南芥类似花药不开裂雄性不育突变体(like-defective in anther de-hiscence,ldad),其果荚干瘪,花药不能开裂且花粉败育。遗传分析表明,突变体的表型受2个隐性基因控制;细胞学观察发现,在花药发育过程中伴随着小孢子的降解;通过图位克隆初步对ldad的2个突变位点分别定位,一个定位在1号染色体上SSLP标记F22L4与端粒之间171 kb的区间,另一个定位在5号染色体上SSLP标记T10O8与端粒间150 kb的区间内;生物信息学分析显示此区间内未见育性相关的已知基因。该研究的结果对进一步克隆LDAD1&2基因及探讨其在花药发育中的功能具有重要意义。
A male sterile mutant like-defective in anther dehiscence, ldad, was isolated by enthyl-methane sulfonate （EMS） mutagenesis strategy. Genetic analysis indicated the mutation pbenotype was controlled by two recessive nuclear genes. The mutant had empty siliques and indehiscent anthers with shrink pollen grains. Cytological observation showed the microspore degradation occurred during anther development. The two mutation loci were mapped to the region of 171 kb between top-telomere and SSLP marker F22L4 on Chr. 1,and the region of 150 kb flanked between top-telomere and SSLP marker T1008 on Chr. 5, respectively. According to the result of bio-informatics analysis, there are not previous male sterile genes in these regions. The results provide a basis for further cloning and functional analysis of LDAD1 and LDAD2 during anther development.
出处
《西北植物学报》
CAS
CSCD
北大核心
2011年第10期1969-1975,共7页
Acta Botanica Boreali-Occidentalia Sinica
基金
上海市高校选拔优秀青年教师科研专项
国家自然基金(30870225)
上海市教委分子生理学重点学科
关键词
图位克隆
拟南芥
雄性不育
小孢子
花药
map-based cloning
Arabidopsis thaliana
male sterile
microspore
anther
