中华补血草盐腺发育的解剖学研究

Developmental and Anatomical Studies of the Salt Gland in Limonium sinense

采用叶表皮印痕、扫描电镜以及石蜡切片法对中华补血草的叶片进行解剖研究,观察其成熟盐腺的结构、盐腺的发育与盐腺的密度。结果表明:(1)上表皮的盐腺密度比下表皮的略小。(2)成熟的盐腺由20个细胞构成,中央有4个分泌细胞,每一个分泌细胞外侧又伴有一个长方形的毗邻细胞;向外由2层杯状细胞包围,每一层分别有4个杯状细胞,使盐腺呈近似圆形;盐腺内部靠近叶肉细胞处有4个收集细胞;中央4个分泌细胞顶端的角质层各有一小孔,是盐分泌出的通道。(3)中华补血草盐腺是由一个单独的表皮细胞发育而成,分别经历单细胞时期、2细胞时期、4细胞时期、8细胞时期、16细胞时期和20细胞时期的不同发育阶段。

The leaf anatomical structure of Limonium sinense was studied by leaf epidermis segregation,SEM and paraffin method,especially for observing the salt gland structure and the salt gland development.The density of salt glands was measured on the leaf as well.Experimental results showed that：（1）The density of salt glands on the adaxial epidermis was slightly smaller than on the abaxial epidermis.（2）The mature salt gland included 20 cells.There were 4 central secretory cells.Outside the secretory cell was the adjacent cell.The adjacent cells and secretory cells were surrounded by 2 layers of goblet cells containing 4 inside cells and 4 outside cells.Behind them there were 4 big cells called collecting cell which was closest to mesophyll cells.Secretory cell had a small hole on the center of cuticle.Salt released from the holes of salt gland.（3）The salt gland of Limonium sinense developed from a single epidermal cell.After that,it went through 5 stages which were called as 2 cells stage,4 cells stage,8 cells stage,16 cells stage and 20 cells stage,respectively.