一串红染色体制片技术优化与计数

Optimization of Chromosome Sectioning and Chromosome Counting of Salvia splendens

以一串红商品种‘展望红’萌发种子根尖、幼苗茎尖生长点以及嫩叶为实验材料,利用常规压片法比较不同材料、不同预处理液及预处理时间对一串红染色体制片的影响,探索实验预处理条件。然后利用去壁低渗法对一串红4个商品种和一串红株型突变体及其野生型进行染色体计数。实验结果显示：以一串红萌发种子的根尖为实验材料,用0.002 mol/L 8-羟基喹啉预处理3~4 h压片所得染色体效果最好;分别观察6个供试品种（系）分散良好、清晰的30个分裂相细胞,86.7%及以上的细胞染色体数目为2n=44。研究表明,一串红株型突变体与野生型及4个商品种在染色体数目上没有差异。

The roots of germinating seeds,stem tips and tender leaves of Salvia splendens commercial variety,＇Vista Red＇ were used to study the effect of different plant materials,pretreatment medicaments and pretreatment time on the slide-preparing of the chromosomes by conventional squashing technique,exploring the pretreatment conditions of the experiment.We have also statistics for the chromosomes number of the plant type mutant,wild type and 4 commercial varieties of Salvia splendens by the technique of wall degradation and hypotonic treatment.The results showed that the roots of germinating seeds were pretreated with 0.002 mol/L 8-hydroxyquinoline for 3~4 h performed best in the sectioning;30 metaphase cells were observed for each variety.We found that chromosome numbers are 2n=44 in 86.7% and above cells.There are no differences in the chromosome numbers of the plant type mutant,wild type and 4 commercial varieties of Salvia splendens.