S100A4与MMP-9在喉鳞癌中的表达和相关性研究

Expression and Correlation of S100A4 and MMP-9 in Laryngeal Squamous Cell Carcinoma

目的:分析正常喉组织、喉鳞癌组织中S100A4和MMP-9的表达及临床意义,探讨其在喉鳞癌发生发展中的作用及相关性。方法:采用免疫组织化学SP法检测S100A4和MMP-9蛋白的表达并分析二者的相关性及其与临床病理因素的关系;采用Western blot法检测S100A4和MMP-9蛋白的表达。结果:免疫组化结果表明S100A4和MMP-9蛋白在癌旁喉组织和喉鳞癌中的阳性表达率均呈升高趋势,分别为8.6%和14.3%,63.8%和71.3%,并且二者表达均与喉鳞癌淋巴结转移相关。统计学结果显示80例喉鳞癌组织中,S100A4和MMP-9蛋白表达呈正相关(P<0.01,r=0.440)。Western blot结果表明S100A4和MMP-9蛋白在喉鳞癌组织中表达高于癌旁喉组织。在喉癌细胞系Hep-2中干扰S100A4蛋白的表达后,MMP-9的蛋白水平下调。结论:S100A4和MMP-9在喉鳞癌发生发展中均存在异常,并且S100A4基因抑制后能够显著下调MMP-9的表达。

Objective： To investigate the expression and significance of both S100A4 and MMP-9 in normal laryngeal tissues and laryngeal squamous cell carcinoma, and to discuss the roles of S100A4 and MMP-9 and their correlation in the process of laryngeal squamous cell carcinogenesis. Methods： The expressions of SI00A4 and MMP-9 were determined using immunohistochemical S-P method. The relationship between the expressions of S 100A4 and MMP-9 and their clinical pathological features were analyzed using the Western blot method. Result： The expression of S100A4 and MMP-9 significantly increased in laryngeal squamous cell carcinoma and was correlated with lymphatic metastasis. The immunohistochemical results showed an increasing tendency in the positive rate of S100A4 and MMP-9 expressions from normal laryngeal tissues （ 8.6% and 14.3%, respectively ） as well as from laryngeal squamous cell carcinoma （ 63.8% and 71.3%, respectively ）. The Spearman＇s correlation test revealed that the S100A4 expression was significantly positively associated with MMP-9 expression in the 80 laryngeal squamous cell carcinoma specimens （ P 〈0.01, rs = 0.440 ）. Western blot results showed that expression of S 100A4 and MMP-9 protein in laryngeal squamous cell carcinoma tissues was significantly higher than that in the non-tumorous counterparts, and the expression of MMP-9 protein in Hep-2 cells decreased after restraining the S 100A4 protein. Conclusion： Both S 100A4 and MMP-9 were abnormal in the development of laryngeal squamous cell carcinogenesis, and S 100A4 gene suppression significantly decreased the expression of MMP-9.