胃癌组织中钙粘附蛋白-E和S100A2表达与其临床生物学行为关系的研究

Expressions of E-cadherin and S100A2 in Gastric Cancer Tissues and Their Clinical Relationship

目的:研究胃癌组织中粘附蛋白-E(E-cadherin)和S100A2的表达,及其与胃癌的临床生物学行为的关系。方法:采用半定量RT-PCR方法及免疫组织化学(IHC)法,检测辽宁省肿瘤医院2006年7至12月收治的不同分期胃癌组织标本,配对癌旁组织及正常胃黏膜组织检测E-cadherin和S100A2的表达情况,分析与肿瘤临床特征的相关性及其在胃癌进展中的作用。结果:E-cadherin和S100A2扩增产物长度分别为487 bp和362 bp。E-cadherin及S100A2基因在正常对照组胃黏膜织表达阳性率为100%,不同分期胃癌组织基因表达的阳性率均下降。两者在正常胃黏膜中蛋白表达阳性率为100%,在胃癌组织中蛋白表达阳性率下降。两者在胃癌中表达的一致性较高,在胃癌的大体分型、分化程度、浸润深度及淋巴结转移等临床特征中,蛋白阳性表达率差异有统计学意义。结论:E-cadherin和S100A2在胃癌组织中表达均下降,是肿瘤抑制因子,其表达与胃癌的分化、转移、侵袭呈负相关。

Objective： To investigate and discuss the relationship between the expressions of E-cadherin and S100A2 genes in gastric cancer tissues, as well as their clinical characters. Methods： Semi-quantitative RT-PCR and immunohistochemistry were performed in the tumor tissues with various grades, paracancerous, and normal gastric tissues to observe the functions of E-cadherin and S100A2 in gastric cancer tissues. Sixty-four cases of tumor tissues with various grades were collected from July 2006 to December 2006 in the Liaoning Province Tumor Hospital, and 15 gastric para-neoplastic and normal gastric tissues were collected as controls. Sta- tistical methods were used to analyze the relationship between the clinical characters of E-cadherin and S 100A2 and their roles in tumor progression. Results： The length of the amplified products of E-cadherin and S100A2 was 487 bp and 362 bp, respectively. The posi- tive expression of E-cadherin and S 100A2 genes in normal gastric tissues was both 100%, whereas the expression was lower in gastric cancer tissues. Moreover, same results were obtained in the positive expression of E-cadherin and S 100A2 proteins. The expression of E-cadherin and S 100A2 had high consistency in gastric cancer tissues. The positive expression of E-cadherin and S 100A2 proteins was statistically significant in clinical characters, such as gross type, differentiation, invasion depth, and lymph node metastasis. Conclusion： E-cadherin and S100A2 are tumor-inhibiting factors, and their expression in gastric cancer tissues decreased. The expression was negatively correlated with to tumor differentiation grade, lymph node metastasis, and invasion grade.