卵巢癌铂类耐药细胞株与敏感株间差异蛋白的筛选

Screening the differential proteins between platinum-resistant and platinum-sensitive cell line of ovarian cancer

目的:筛选卵巢癌铂类耐药细胞和敏感细胞间的差异蛋白质。方法:提取卵巢癌耐卡铂细胞SKOV3/CB和亲本细胞SKOV3的总蛋白,用二维液相色谱技术(ProtemeLabTM PF-2D)分离、筛选差异蛋白,用电喷雾离子化串联质谱(ESI-MS/MS)分析鉴定蛋白质。结果:共分离筛选出差异蛋白54个,其中SK-OV3/CB表达上调的34个,SKOV3表达上调的20个。初步鉴定出5个SKOV3/CB表达上调的差异蛋白,即硫氧还原蛋白(thioredoxin,Trx)、Myosin regulatorylight polypeptide 9(MYL9)、AX887247NID、stanniocalcin homolog、human regu-lator of G-protein signaling 5(RGS-5)。这些蛋白涉及氧化还原、凋亡调控、甲基化及信号传导等多方面。结论:卵巢癌铂类耐药细胞和敏感细胞间存在蛋白表达差异,这些差异蛋白可能通过多种机制介导了卵巢癌对铂类的耐药。

OBJECTIVE：To screen the differential proteins between platinum-resistant and platinum-sensitive cell line of ovarian cancer.METHODS：The total proteins of carboplatin-sensitive（SKOV3） and resistant（SKOV3/CB） human ovarian epithelial cancer cell line were extracted,and the differentially expressed proteins were separated and screened by two-dimensional liquid chromatography.Then the differential proteins were identified by electrospray ionization-tandem mass spectrometry（ESI-MS/MS）.RESULTS：A total of 54 differential proteins were isolated and screened,in which 34 proteins were up-regulated in SKOV3/CB and 20 proteins were up-regulated in SKOV3.And 5 differential proteins up-regulated in SKOV3/CB cells were identified and well characterized by bioinformation,including Thioredoxin（Trx）,Myosin regulatory light polypeptide 9（MYL9）,AX887247 NID,stanniocalcin homolog and human regulator of G-protein signaling 5（RGS-5）.These proteins were associated with oxidation and deoxidization,apoptosis,methylation and signal transduction.CONCLUSION：There exists difference in protein expression between platinum-resistant and platinum-sensitive cell line of ovarian cancer,and these differential proteins may induce the platinum resistance in ovarian cancer through the multiple mechanism.