FISH技术检测卵巢上皮性肿瘤组织CCND1表达及临床意义

Fluorescence in situ hybridization analysis of expression and clinic significance of CCND1 gene in epithelial ovarian neoplasms

目的:探讨荧光原位杂交技术(FISH)检测卵巢上皮性肿瘤中CCND1基因的表达及其临床意义。方法:应用FISH技术检测45例卵巢正常组织和113例卵巢上皮性肿瘤中CCND1基因的表达。结果:CCND1基因在正常卵巢组织、卵巢良性肿瘤、交界性肿瘤及恶性肿瘤中的扩增表达率分别为11.1%、33.3%、43.5%和66.1%,恶性组的表达扩增率明显高于其他组,差异有统计学意义,P<0.01;卵巢上皮性癌组织中CCND1基因的表达扩增率与病理分级、临床病理分期及癌组织转移明显相关,差异有统计学意义(P<0.05),但与年龄、病理学类型无相关性,P>0.05。结论:CCND1基因的扩增在卵巢肿瘤的发生发展中起重要作用,且与卵巢癌的病理分级、临床分期及癌组织的转移有关,可用以预测肿瘤患者的预后。

OBJECTIVE：To investigation the CCND1 gene and its clinical significance in epithelial ovarian tumors.METHODS：The expression of CCND1 gene in 113 cases of epithelial ovarian tumors and 40 cases of normal ovarian tissue were detected by fluorescence in situ hybridization.RESULTS：Positive expression rates of the CCND1 gene in normal ovarian tissue,benign tumor,borderline tumor and ovarian cancer were separately 11.1%,33.3%,43.5%,66.1%.Compared with other groups,positive expression rate of ovarian cancer was higher（P0.01）.The positive expression rate of CCDN1 gene were significantly related to histological grade,clinicopathological stage and metastasis of ovarian carcinoma（P0.05）,and it was non-significant correlation with the patient＇s age and pathological types（P0.05）.CONCLUSIONS：The CCDN1 gene plays a role in the development of ovarian cancer.The positive expression rate of it may serve as a new prognostic maker for ovarian tumor.