摘要
筛选到一株高效生产多糖的YX菌,油田先导试验证明该菌可以用于微生物调剖提高原油采收率(Microbial Enhanced Oil Recovery,MEOR).经16SrDNA分析鉴定,YX菌属于肠内杆菌属(Enterobacter.),为跟踪监测其动态信息,构建表达绿色荧光蛋白(green fluorescent protein,GFP)的重组质粒pET-30a(+)-EGFP,利用CaCl2法将其转化至YX菌,利用荧光显微镜可以观察到重组YX(GFP)工程菌的绿色荧光.结果表明YX菌可以被绿色荧光蛋白基因标记,用于微生物采油研究.
A strain（named YX） isolated from Shengli Oil Field,capable of producing polysaccharides for Microbial Enhanced Oil Recovery（MEOR） ,has been identified as a member of Enterobacter sp.through 16SrDNA sequence analysis.Recombinant plasmid of pET-30a（＋） -EGFP has been transferred into natural strain YX using cold CaCl2 method,and green fluorescent expressed by engineered strain YX containing green fluorescent protein（GFP） gene has been observed and monitored.This study provides a new approach that can track and monitor the dynamic data of a goal strain for enhanced oil recovery.
出处
《徐州工程学院学报（自然科学版）》
CAS
2011年第4期56-60,共5页
Journal of Xuzhou Institute of Technology(Natural Sciences Edition)
基金
国家高科技研究发展计划(863计划)项目(2009AA063504)
关键词
微生物采油菌
16S
rDNA鉴定
绿色荧光蛋白
重组质粒
Microbial Enhanced Oil Recovery（MEOR） strain
16S rDNA
green fluorescent protein（GFP）
recombinant plasmid
