转Btcry3A抗虫基因杨树中毒蛋白的时空表达

Temporal and Spatial Expression of Bt Toxic Protein in Transgenic Btcry3A Hybrid Poplar 741

转Btcry3A基因741杨（Populus×aldatomentosacl.741）目前已进入中间试验林阶段,近几年来一些学者对转Btcry3A基因741杨不同株系的抗虫性进行了一系列的室内和野外测定,研究了转Bt基因741杨对目标害虫产卵、生长发育及致死率的影响（甄志先等,2007;王彦平等,2008;王永芳等,2002）。测定结果表明转基因741杨不同株系对目标害虫表现出一定抗性。

We further studied strain PCC1 of transgenic Btcry3A poplar 741,which had stable resistance to the pest,and examined the Bt toxic and soluble proteins of twig xylem,leaf and root at different developmental time.The results showed that there was no difference in soluble protein content between transgenic and CK strain,but the soluble proteins of the root and the twig xylem and leaves of different parts in the crown presented a certain regularity.The proteins in twig xylem and roots increased all the time while the proteins in the leaf first decreased and then increased in the whole growing seasons.The expression of Bt toxic protein in xylem increased from upper to lower crown and the expression in leaves decreased from upper to lower crown;the expression of Bt toxic protein in xylem first increased and then decreased with the peak in September.However the expression in leaves and roots did not exhibit a certain regularity with the time on.