两种不同治法对肝纤维化大鼠肝组织TGF-β_1及其mRNA表达的影响

Two different therapies for liver fibrosis in rat liver tissue on mRNA expression of TGF-β_1 and its impact

目的:研究两种不同中药复方对CCl4致肝纤维化大鼠肝组织转化生长因子-β1及mRNA表达的影响,探讨不同中医治法防治肝纤维化的作用机制。方法:皮下注射法制备CCl4大鼠肝纤维化模型,给予两种不同中药复方煎剂灌胃,观察大鼠肝组织匀浆TGF-β1浓度及TGF-β1mRNA表达。结果:与空白对照组比较,模型组大鼠TGF-β1的mRNA和蛋白水平均明显异常表达;治疗1组、2组均可降低肝组织匀浆TGF-β1含量及TGF-β1mRNA表达水平,与模型组比较有显著性差异(P<0.01或P<0.05),尤以滋肾清肝饮组疗效更加明显。结论:滋肾清肝化瘀法与滋肾化瘀法皆可有效防治CCl4大鼠肝纤维化,配伍清热利湿解毒中药后作用可能更加明显。

Objective： To study two different Chinese herbal compound on CCl4-induced liver fibrosis in rat liver tissue and transforming growth factor-ill mRNA expression, and to explore different treatment methods in the prevention and treatment of liver fibrosis in rats. Methods： Preparation of subcutaneous injection of CCl4 in rat liver fibrosis model, two different Chinese herbal compound decoction given orally, rat liver homogenates the concentration of TGF-β1 and TGF-β1 mRNA expression. Results： Compared with control rats, the TGF-β1 mRNA and protein expression were significantly abnormal; treatment group 1, group 2 can reduce the liver tissue content of TGF-β1 and TGF-β1 mRNA expression levels, compared with model group with significant difference （P〈0.01 or P〈0.05）, especially Kidney Liver drinking group effect more pronounced. Conclusion： Kidney Liver and Kidney Stasis Stasis Jieke effective prevention and treatment of liver fibrosis in CCl4, clearing away heat and detoxifying herbs compatibility after effects may be more apparent.