浓缩糖蜜酒精废液中蛋白质的提取工艺研究

Study on the extraction of protein from concentrated Wastewater from Cane-Sugar Molasses Alcohol

以浓缩糖蜜酒精废液为原料，测定废液中蛋白质在等电点处的最佳pH值，分别研究了碱溶酸沉法和醇-碱法提取废液中的蛋白质。研究结果表明：浓缩糖蜜酒精废液中蛋白质的等电点为pI=4．2；使用醇-碱法蛋白质的提取量高于碱溶酸沉法，醇-碱法提取蛋白质的最佳工艺条件是以料液比1：36、醇-碱比为1：3．6、在常温下提取44min，在此条件下蛋白质提取率为39．7％。

Crude protein is extracted from concentrated wastewater from cane-sugar molasses alcohol using alkali-solution and acid-isolation solution and alcohol-alkali solution.The results showed the pI of protein in concentrated wastewater of cane-sugar molasses alcohol was 4.2.The extraction of protein with alcohol-alkali solution was better than that with alkali-solution and acid-isolation solution.The optimum conditions with alcohol-alkali solution were as follows：the ratio of concentrated wastewater of cane-sugar molasses alcohol and extraction solvent is 1：36, the ratio of alcohol and alkali is 1：3.6,the time of extration is 44 min. ,the extraction of protein accounts for about 39.7% under the optimum conditions by the kjeldahl method.