胃转流手术后GK大鼠胃肠道GLP-1表达变化的研究

EFFECTS OF GBP SURGERY ON THE EXPRESSIONS OF GLP-1 IN GK RAT GASTROINTESTINAL TRACT

目的研究胃转流(GBP)手术对GK大鼠胃肠道GLP-1表达的影响。方法将30只体重240～260g GK大鼠随机分成3组(每组10只):①对照组:正常GK大鼠,无任何干预;②假手术组:行胃窦部离断后吻合术;③胃转流手术组:将胃窦部切断,同时在十二指肠悬韧带下方8cm处切断小肠,将胃窦近断端与上提的小肠远断端行端端吻合,小肠的近断端与胃肠吻合口下方8cm的小肠行端侧吻合。动物到达存活时间点后取材,应用免疫组织化学S-P法和图像分析技术监测GK大鼠胰腺及胃肠道各段GLP-1的表达。结果 GBP手术组胃肠吻合口下方回肠远端GLP-1表达明显高于对照组和假手术组(P<0.05)。结论食物转流的效应部位可能位于回肠远端,GBP手术可以刺激回肠远端的GLP-1表达增多,促进胰腺细胞β增殖,胰岛素分泌增加。

Objective To investigate the changes of GLP-1 expressions in GK rat gastrointestinal tract after GBP surgery.Methods 30 males GK rats weighing 240-260g were divided into three groups at random.In the normal control group,GK normal rats showed no interference.In the Sham group,sinus ventriculi and anastomosis were cut off.In the GBP surgery group,sinus ventriculi and the small intestine bellow ligament of duodenum 8 cm of GK rat were cut off,the distal end gastric and the proximal end of the small intestine was to end-to-end anastomosis,below 8cm of gastric-intestine anastomotic stoma,the distal end of the small intestine and the small intestine was end-to-side anastomosis.The expressions of GLP-1 in the gastrointestinal tract were examined by immunohistochemistry and image analysis.Results After operation,the expressions of GLP-1 increased on the distal end of ileum compared with the sham group and the control group.Conclusion The effective location of food by-pass may be the distal end of ileum.The results suggest that the expressions of GLP-1 increases in pancreas and gastrointestinal tract after GBP,and the increase of GLP-1 may help to advance βcell multiplication and increase insulin secretion.