5L立瓶发酵制备益生菌直投式发酵剂及应用试验

Preparation of DVS for probiotics incubated in 5 L roller bottle and application test

对长双歧杆菌、保加利亚乳杆菌、嗜热乳酸链球菌在5 L立瓶中分别进行培养,确定了各自的发酵最适收获期,菌体收集后经制备所获得冷冻浓缩直投式发酵剂活菌数大于1010mL-1,冷冻干燥直投式发酵剂活菌数大于1011g-1。以制备的冷冻浓缩、冷冻干燥直投式发酵剂,进行牛乳发酵应用试验,生产的益生菌酸奶风味纯正、品质良好。

Bifidobacterium Iongum, Lactobacillus bulgaricus and Streptococcus thermophilus was incubated in 5 L roller bottle, respectively. Fermentation optimal harvest time of the three probiotics were determined. The viable cell counts of freeze-concentration DVS 〉10^10 CFU/mL. The viable cell counts of freeze-drying DVS 〉1011 CFU/g. Milk was fermented by freeze-concentration DVS or freeze-drying DVS, Results showed that the probiotic yogurt made from milk was right taste, good quality.