固相分散萃取-液相色谱法测定奶酪中3种雌激素

Determination of three estrogens in cheese by Solid-Phase Dispersive Extraction Coupled with High Performance Liquid Chromatography

建立了同时测定奶酪中炔雌醇、己烯雌酚和双烯雌酚三种雌激素的高效液相色谱分析方法。采用固相分散萃取技术处理样品,弗罗里硅土为分散剂、乙腈为萃取剂,经氮气流浓缩,反相色谱分离,紫外230 nm检测得出结果。3种激素回收率分别为89.16%,98.22%和83.22%,相对标准偏差2.98%,1.20%和2.75%,方法检出限分别为0.25,0.25和0.12μg/g。对市售奶酪进行了实际测定,三种雌激素均未检出。

An efficient method for analyzing three estrogens （ethinyloestradiol , diethylstilbestrol; dienestrol） in cheese by high pertormance liquid chromatography was developed. The solid-phase dispersion extraction technology was used for sample treatment The dispersant was Florisil, and the extractant was acetonitrile .The extract was concentrated by nitrogen flow. three estrogens were separated and detected by the reverse chromatography at uv 230 nm to obtain the result. Three estrogens recoveries are 89.16%, 98.22% and 83.22%, respectively. Relative standard deviations are 2.98%,1.20% and 2.75%, the detection limit are 0.25 μg/g, 0.25 txg/g and 0.12 μg/g, respectively. The sold cheese is determined, and the three kinds of estrogen is not detected in here.