半乳糖凝集素-3在病毒性心肌炎小鼠心肌组织中的动态变化及临床意义

Dynamic Change of Galectin-3 in Myocardium Tissue of Mice with Viral Myocarditis and Its Clinical Significance

目的探讨半乳糖凝集素-3(Gal-3)在小鼠柯萨奇病毒B3(CVB3)所致病毒性心肌炎(VMC)中的表达及其临床意义。方法选取58只健康雄性Balb/C小鼠,随机分为VMC组(n=50)和对照组(n=8)。VMC组小鼠腹腔接种半数组织培养感染剂量(TCID50)为10-5L-1的Nancy株CVB3病毒液0.1 mL,分别于接种第7、10、14、28天随机取8只处死;对照组小鼠腹腔接种不含病毒的eagle氏液0.1 mL,于第28天处死。光镜下观察小鼠心肌组织的病理变化并计算心肌病理积分;运用实时荧光定量PCR检测心肌组织中Gal-3 mRNA的表达,应用ELISA法检测外周血Gal-3水平,并与心肌病理积分作直线相关性分析。结果 1.与对照组小鼠比较,VMC组小鼠心肌组织Gal-3 mRNA的表达在第7天(0.91±0.64 vs 10.09±2.78)、第10天(0.91±0.64 vs 12.49±6.08)和第14天(0.91±0.64 vs 2.86±2.90)均明显增高(Pa<0.05)。2.与对照组小鼠外周Gal-3水平(5.27±1.11)比较,VMC组小鼠外周血Gal-3水平在第7天(9.70±2.39)、第10天(8.98±3.03)和第14天(7.73±1.88)也均明显增高(Pa<0.05)。3.小鼠心肌组织中Gal-3 mRNA与外周血中Gal-3表达水平均与心肌病理积分呈直线正相关(r=0.77、0.69,Pa<0.05)。结论 Gal-3参与VMC的发生与发展,且与VMC的病情严重程度有关,可作为VMC新的检测指标。

Objective To investigate the dynamic change of Galectin - 3 （ Gal - 3 ） in mice with coxsackie virus B3 （ CVB3 ） induced myocarditis and its clinical significance in viral myocarditis（VMC）. Methods A total of 58 healthy male Balb/C mice were randomly divided into VMC group （n = 50） and control group （n = 8 ）. The mice in VMC group were treated with 0. 1 mL 10-5 L-1 CVB3, and 8 cases of infected mice were respectively sacrificed on the 7thh day, 10th day, 14thday and 28th day ; while the mice in control group were inoculated intraperitoneally with O. 1 mL eagle reagent and sacrificed on the 28th day. The myocardial pathological changes were observed through light microscope and histopathological scores were calculated： In addition, the expressions of Gal - 3 in myocardial and serum were respectively detected by means of real - time quantitative - polymerase chain reaction and enzyme - linked immunosorbent assay, and then linear correlation analysis with myocardial histopathology scores was conducted. Results 1. Compared with control group, the expression of Gal - 3 mRNA in myocardial in VMC group obviously increased on the 7th day（0.91 ±0.64 vs 10.09 ± 2.78,P 〈0.05） ,10th day（0.91 s0.64 vs 12.49 ±6.08,P 〈 0. 05 ） and 14th day （0.91 ±0.64 vs 2.86 ± 2.90, P 〈 0.05 ）. 2. The serum concentration of Gal - 3 in VMC group obviously increased on the 7th day （9.70± 2.39）, 10th day （ 8.98 ± 3.03 ） and 14th day （ 7.73 ± 1.88 ） compared with control group （ 5.27 ± 1.11 ） （ P, 〈 0.05 ）. 3. There were significantly positive correlations between the expression of Gal - 3 mRNA in myocardial and serum with myocardial histopathological scores respectively （ r = 0.77,0.69 ; P, 〈 0.05 ）. Conclusions Gal - 3 could be involved in the pathogenesis and development of VMC and related with the extent of VMC. It might serve as a new detection mark for VMC.