复方血栓通片中人参皂苷Rb1与Rg1在大鼠体内的药代动力学研究

Pharmacokinetic Behavior of Ginsenoside Rg1 and Rb1 in Fufang Xueshuantong Tablets in Rats

目的考察大鼠灌胃口服复方血栓通片后人参皂苷Rb1与Rg1在大鼠体内的药代动力学特征。方法通过建立测定血浆中人参皂苷Rg1与Rb1的液质联用色谱法(LC-MS),测定8只大鼠灌胃给1 200mg.kg-1的复方血栓通片后的血药浓度,通过DAS软件进行药代动力学统计分析。结果大鼠灌胃给1 200mg.kg-1的复方血栓通片后,血浆中人参皂苷Rb1与Rg1达峰时间均快,分别为1.02h与1.05h;与人参皂苷Rb1相比,人参皂苷Rg1原形在胃肠吸收较差,生物利用度低;人参皂苷Rb1半衰期长[(16.45±3.17)h]而人参皂苷Rg1半衰期短[(4.33±1.34)h]。结论复方血栓通片中人参皂苷Rb1与Rg1的药代动力学研究对含该组分的新药研发和制剂研究等有一定的意义。

Objective To investigate the pharmacokinetic behavior of ginsenoside Rg1 and Rb1 after intragastric administration of Fufang Xueshuantong tablets in rats. Methods The LC-MS was established to determine the blood drug concentrations of ginsenoside Rg1 and Rb1 after intragastric administration of Fufang Xueshuantong tablets （1 200 mg · kg^-1） in 8 rats. Statistical analysis of pharmacokinetic data was carried out by DAS. Results After intragastric administration of Fufang Xueshuantong tablets, plasma concentrations of ginsenoside Rg1 and Rb1 reached maximum values quickly（1.02 h,1.05 h）. The absorption of Rg1 was poorer and the bioavailability was lower as compared to Rb1. Moreover, the half life of Rb1 was longer than that of Rg1 [（16.45±3.17） hours vs （4.33± 1.34） hours]. Conclusion The pharmacokinetic behavior of ginsenoside Rg1 and Rb1 in Fufang Xueshuantong tablets has positive significance in the study of new drugs and preparations containing ginsenoside Rg1 and Rb1.