厌氧氨氧化细菌的分子生物学鉴定

Identification of ANAMMOX Bacterium by Molecular Biology

采用分子生物学方法从厌氧氨氧化污泥中提取细菌总DNA,使用特异性引物进行PCR扩增。扩增产物经过克隆、测序后,得到厌氧氨氧化菌部分16S rDNA序列(长度为838 bp)。将得到的序列构建系统发育树进行分析。结果表明,厌氧氨氧化污泥中的厌氧氨氧化细菌与Anaerobic Ammonium-Oxidizing Planctomycete(AJ131819)细菌在进化上关系最近,并将该类细菌暂命名为Anaerobic Ammonium-Oxidizing Planctomycete ASBBR Gene。

The anaerobic ammonium oxidation（ANAMMOX） sludge was enriched in an anaerobic sequencing biofilm batch reactor（ASBBR）.Total DNA of the bacteria was extracted from the anaerobic ammonium-oxidizing sludge by a molecular biology method,and was then amplified by the polymerase chain reaction（PCR）with a specific primer.After the PCR product was cloned and sequenced,the partial 16S rDNA sequence of ANAMMOX bacterium（length：838 bp）was obtained,and the sequence was analyzed by the phylogenetic tree.The results show that the cultivated ANAMMOX bacterium greatly closes to the anaerobic ammonium-oxidizing planctomycete（AJ131819）,thus the bacterium is named temporarily as anaerobic ammonium-oxidizing planctomycete ASBBR gene.