鲜苎麻叶中总酚酸提取工艺研究

Optimization of Extraction Technology of Total Phenolic Acid from Ramie

为了研究鲜苎麻叶中总酚酸的提取分离工艺,以惰性气体饱和的氢氧化钠溶液为溶剂,提取鲜苎麻叶中的总酚酸,以AB-8大孔树脂进行分离纯化,并采用均匀设计法优化提取工艺。惰性气体保护提取分离苎麻叶中总酚酸工艺的适宜条件为,采用闪蒸提取器提取,提取次数2次,提取时间1min,碱液浓度1.0mol.L-1,料液比1:8,提取液调节pH3.5,取上清液用大孔树脂AB-8柱层析分离,每100ml大孔树脂上样量为2g,洗脱液为80%乙醇溶液。总酚酸粗品得率(占干燥叶片百分比)为4.225%;总酚酸在干燥叶片中的含量为0.3681%。结果显示,该工艺采用碱水提取,避免使用有机溶剂,有效降低了提取成本;同时采用惰性气体保护,避免了提取过程中酚酸类成分的氧化,简便可行。

To design different extraction and separation technology of total polyphenol in Boehmeria nivea （Linn.） Gaudich. To extract fresh ramie leaves of total phenolic acids with an inert gas saturated sodium hydroxide solution as solvent. Purified by AB-8 macroporous resin and optimized extraction technology by uniform design, the best conditions of extraction and separation total phenolic acids from ramie leaves protected with Inert gas are as follows, using braising extraction device to extract 2 times, 1 minute, alkali concentration, 1.0 L^-1 mol, material： liquid , 1 ： 8, pH3.5, than separating with macroporous resin AB-8 column chromatography , adding 2 g sample for every 100 ml macroporous resin, 80% alcohol solution as the eluent. Total phenolic acids crude products yield （the percentage of dry out） for 4. 225%; the content of total phenolic acids in dry out is 0. 3681%. Result is this process-using buck for extraction, so as to avoid using organic solvent for extraction, effectively reduce the cost of extraction; At the same time, avoiding oxidation of the phenolic acids composition in extraction process with inert gases protection. The method is simple and feasible.