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肾癌组织中Tim-3基因的甲基化分析 被引量：2

Abnormal methylation of Tim-3 gene in renal carcinoma
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摘要 目的分析肾癌组织中Tim-3基因启动子的甲基化情况,探讨Tim-3甲基化在肾癌发生中的可能作用。方法采用甲基化特异性PCR(MSP)检测20例肾癌组织和3例癌旁组织中Tim-3基因启动子甲基化状态,分析检测结果。结果肾癌组织中有11例(55%)检测到了Tim-3基因甲基化表达,相应癌旁组织中没有检测到Tim-3基因甲基化表达。结论肾癌组织中Tim-3基因启动子发生甲基化,Tim-3基因甲基化可能与肾癌的发生发展有关。 Objective To investigate the methylation level of the promoter of Tim-3 gene in the renal carcinoma tissues and to assess its role in the renal carcinoma genesis.Methods Methylation specific PCR （MSP） method was used to analysis the methylation status of the promoter of Tim-3 gene in the renal carcinoma tissues of 21 patients with renal carcinoma and 3 corresponding adjacent normal tissues.Results Tim-3 gene methylation found in 11 （52.4%）） of the renal carcinoma tissues and no abnormal Tim-3 gene methylation found in the 3 adjacent normal tissues.Conclusions Hypermethylation of Tim-3 gene promoter is present in the renal carcinoma tissues,suggesting that hypermethylation of Tim-3 maybe associated with the tumorigenesis of renal carcinoma.
作者 郑伏甫 陈恩静 李晓飞 邓春华 戴宇平 丘少鹏
机构地区 中山大学附属第一医院泌尿外科 中山大学附属第一医院内科
出处 《现代泌尿生殖肿瘤杂志》 2011年第6期352-353,355,共3页 Journal of Contemporary Urologic and Reproductive Oncology
基金 广东省科技计划项目基金(2010B080701059)
关键词 肾肿瘤 DNA甲基化 MSP TIM-3 Kidney neoplasms DNA methylation MSP Tim-3
分类号 R737.11 [医药卫生—肿瘤]
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参考文献16

  • 1Dobrovic A,Simpfendorfer D.Methylation of the BRCA1 gene in sporadic breast cancer[J].Cancer Res,1997,57(16):3347-3350.
  • 2Collins N,Wooster R,Stratton MR.Absence of methylation of CpG dinucleotides within the promoter of the breast cancer susceptibility gene BRCA2 in normal tissues and in breast and ovarian cancers[J].Br J Cancer,1997,76(9):1150-1156.
  • 3Corn PG,Kuerbitz SJ,van Noesel MM,et al.Transcriptional silencing of the p73 gene in acute lymphoblastic leukemia and Burkitt's lymphoma is associated with 5' CpG island methylation[J].Cancer Res,1999,59(14):3352-3356.
  • 4Esteller M,Hamilton SR,Burger PC,et al.Inactivation of the DNA repair gene O6-methylguanine-DNA methyltransferase by promoter hypermethylation is a common event in primary human neoplasia[J].Cancer Res,1999,59(4):793-797.
  • 5黄琼晓,金帆,黄荷凤.DNA甲基化的研究方法学[J].国外医学（遗传学分册）,2004,27(6):354-358. 被引量：17
  • 6DeClerck YA.,Perez N,Shimada H,et al.Inhibition of invasion and metastasis in cells transfected with an inhibitor of metalloproteinases[J].Cancer Res,1992,52(3):701-708.
  • 7Chambers AF,Matrisian LM.Changing views of the role of matrix metalloproteinases in metastasis[J].J Natl Cancer Inst,1997,89(17):1260-1270.
  • 8Gomez DE,Alonso DF,Yoshiji H,et al.Tissue inhibitors of metalloproteinases:structure,regulation and biological functions[J].Eur J Cell Biol,1997,74(2):111-122.
  • 9Anand-Apte B,Pepper MS,Voest E,et al.Inhibition of angiogenesis by tissue inhibitor of metalloproteinase-3[J].Invest Ophthalmol Vis Sci,1997,38(5):817-823.
  • 10Bian J,Wang Y,Smith MR,et al.Suppression of in vivo tumor growth and induction of suspension cell death by tissue inhibitor of metalloproteinases (TIMP)-3[J].Carcinogenesis,1996,17(9):1805-1811.

共引文献16

同被引文献32

  • 1Jemal A, Siegel R, Xu J, et al. Cancer statistics [J]. CA Can- cer J Clin, 2010,60(5) :277 -300.
  • 2Sugiyama Y, Masumori N, Fukuta F, et al. Influence of isoflavone intake and equol-producing intestinal flora on prostate cancer risk [J]. Asian Pac J Cancer Prey, 2013, 14( 1 ) : 1-4.
  • 3Xu LW, Qian M, Jia RP, et al. Expression and significance of microsomal prostaglandin synthasc-I (mPGES-1) and Beclin-I in the development of prostate cancer [J]. Asian Pac J Cancer Prey, 2012, 13(4) : 1639-1644.
  • 4Zhuang X, Zhang X, Xia X, et al. Eetopie expression of TIM-3 in lung eaneers: a potential independenl prognostie factor for patients with NSCLC [J]. Am J Clin Pathnl, 2012, 137(6) :978 -985.
  • 5Wiener Z, Kohalmi B, Pocza P, et al. TIM-3 is expressed in melanoma cells and is upregulated in TGF-beta stimulated mast cells [J]. J Invest Dermalol, 2007, 127(4) :906-914.
  • 6Sakuishi K, Apetoh L, Sullivan JM, et al. Targeting TIM-3 and PD-1 pathways to reverse T cell exhaustion and restore anti-tu- mor immunity [j]. J Exp Med, 2010, 207(10) :2187-2194.
  • 7Foureade J, Sun Z, Benallaoua M, et al. Upregulation of Tim-3 and PD-I expression is associated with tumor antigen-specific CD8+F cell dysfunetion in melanoma patients [J]. J Exp Med, 2010,207(10) :2175-2186.
  • 8Huang X, Bai X, Cao Y, el al. Lymphoma endothelium prefer- entially expresses TIM-3 and facilitates the progression of lym- phoma by mediating immune evasion [J]. J Exp Med, 2010, 207(3) :505-520.
  • 9Abildgaard MO, Borre M, Mortensen MM, et al. Downregula- tion of zinc finger protein 132 in prostate cancer is assoeiated with aberrant promoter hypermethylation and poor prognosis [J]. lnt J Cancer, 2012,130(4) : 885-895.
  • 10Cai C, Wang L, Wu Z, et al. T-cell immunoglobulin- and nmcin-domain- containing molecule 3 gene polymorphisms and renal cell carcinonm [J]. DNA Cell Biol, 2012,31 (7): 1285- 1289.

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现代泌尿生殖肿瘤杂志
2011年 第6期

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