差示分光光度法测定安乃近片的含量

Content Determination of Metamizole Sodium Tablets by Differential Spectrophotometry

目的:建立测定安乃近片含量的新方法。方法:采用差示分光光度法。以0.1mol.L-1盐酸溶液为介质制备参比液,以0.1mol.L-1氢氧化钠溶液为介质制备样品液,通过在波长283nm处,测定2种溶液的差示吸收值直接测定安乃近的含量,并将结果与其他方法(紫外分光光度法、高效液相色谱法和碘量法)比较。结果:安乃近检测浓度线性范围为10.35～51.75μg.L-(1r=0.9998),平均回收率为99.94%,RSD=0.27%(n=5);4种方法含量测定结果基本一致。结论:建立的新方法测定结果准确,方法简便、专属性强,可用于该制剂的含量测定。

OBJECTIVE： To establish new method for the content determination of Metamizole sodium tablets. METHODS： The differential spectrophotometry was adopted. 0.1 mol.L-1 hydrochloric acid and 0.1 mol.L -1 sodium hydroxide were used respec- tively as medium to prepare reference solution and sample solution. The content of metamizole sodium was determined by measur- ing differential absorption value of 2 kinds of solution at wavelength of 283 nm. Results of the study were compared with those of other methods （UV spectrophotometry, HPLC and iodometric method）. RESULTS： The calibration curves showed good linearity in the range of 10.35-51.75 gg.L-1 （r=0.999 8） with an average recovery of 99.94% （RSD=0.27%, n=5）. Results of content de- termination of 4 methods were similar. CONCLUSION： Established method is accurate, simple, specific and suitable for the content determination of Metamizole sodium tablets.