黄连吴茱萸不同配比5种化学成分的大鼠在体肠吸收研究

Rat Intestinal Absorption of Five Chemical Compositions of Match-pair of Coptis-Evodia

目的研究黄连吴茱萸不同配比的在体肠吸收,比较小檗碱等5种化学成分在小肠吸收率及吸收系数的异同。方法采用大鼠在体单向肠灌流模型,以HPLC测定灌流后药液5种化学成分含量及吸收系数,考察黄连吴茱萸不同配比对小檗碱等5种化学成分在小肠吸收和转运的影响。结果随着黄连配比增加,5种化学成分的吸收率有增加趋势,且黄连吴茱萸6∶1时小檗碱、巴马汀、吴茱萸碱及吴茱萸次碱的吸收率均比黄连吴茱萸1∶1和2∶1的吸收率要大,差异有显著性(P<0.01);5种化学成分的Ka值与Kapp值有增加趋势,且黄连吴茱萸6∶1时小檗碱、巴马汀、药根碱及吴茱萸次碱的Ka值均比黄连吴茱萸1∶1、2∶1的Ka值要大,差异有显著性(P<0.01)。结论增加黄连的配伍比例,均有利于黄连和吴茱萸主要化学成分的吸收;黄连吴茱萸的配伍可能在于小肠吸收,且吴茱萸比例越少,黄连吸收越好;黄连比例越多,吴茱萸吸收越好,黄连吴茱萸为6∶1时黄连与吴茱萸的吸收效果最好。

Objective To study the intestinal absorption of different match-pair of Coptis-Evodia,to compare the intestinal absorption rate and absorption coefficient of the five chemical compositions. Methods Single pass perfusion model in rats and HPLC were used to study the concentration and absorption coefficients of the five chemical compositions.The absorption and transport in the small intestine of match-pair of Coptis-Evodia were investigated. Results As the ratio of Coptis increased,the absorption rate of the five chemical components tended to increase,and the berberine,palmatine,evodiamine and rutaecarpine of Coptis-Evodia 6：1 were larger than 1∶1 and 2∶1,the difference was significant（P 0.01）;the Ka Kapp of the five chemical components tended to increase,and the berberine,palmatine,Jatrorrhizine and rutaecarpine of Coptis-Evodia 6∶1 were larger than 1∶1and 2∶1,the difference was significant（P 0.01）. Conclusion Increasing the compatibility of Coptis proportion is benefit to the chemical compositions of Coptis or Evodia.The absorption position of match-pair of Coptis-Evodia may be in the small intestine.