了哥王提取物及其不同提取部位的急性毒性研究

Acute Toxicity of Extract from Indian Stringbush Root and Its Different Extracted Parts

目的观察了哥王提取物及其不同提取部位的急性毒性大小。方法小鼠一次灌胃给予了哥王提取物及其不同提取部位后,观察14 d内各受试样品小鼠的反应情况及死亡情况。记录各样品不同剂量组小鼠死亡只数,用Bliss法计算出该样品的半数致死量(LD50);无小鼠死亡的样品,则测定其最大给药量。结果了哥王提取物的LD50为3.276 g/kg;了哥王石油醚部位的LD50为1.691 g/kg;了哥王醋酸乙酯部位的LD50为1.948 g/kg;了哥王正丁醇部位的最大给药量为24.364 g/kg;了哥王水部位的最大给药量为:32.788 g/kg。结论了哥王提取物的毒性随脂溶性的增大而增大,可以推断其毒性成分主要为脂溶性成分。

Objective To observe the acute toxicity of extract from indian stringbush root and its different extract fractions. Methods Mice were intragastrically administered once with extract from indian stringbush root and its different extracted fraction, the mortality of mice were observed, and the median lethal dose （ LD50 ） of different extracts were calculated with Bliss method. The maximum dose was determined when no mouse died for the extracts. Results The LD50 of extract from indian stringbush root was 3. 276 g/kg; the LD50 of fractions of petroleum ether, ethylacetate part of indian stringbush root were 1. 691 g/kg, 1. 948 g/kg, respectively ; the maximum amounts of n - butanol and water portion were 24. 364 g/kg, 32. 788 g/kg. Conclusion The toxicity of extract increases with liposolubility increasing, which suggests that the toxicity of extract is mainly in lipophilic components.