摘要
首次建立了测定甘草苷的荧光方法。选择了最佳激发和荧光发射波长,考察了乙醇浓度、温度、放置时间、pH值和共存离子对测定的影响。甘草苷的荧光光谱具有良好的稳定性。在最佳实验条件下,其荧光强度与浓度分别在0.04～8.37μg.ml-1范围内呈良好的线性关系,相关系数为0.998 9,检测下限为0.017μg.ml-1。
The liquiritin was determined by fluorescence spectrometry.The effects of ethanol concentration,temperature,placing time,pH values and foreign substances on the determination of the drugs were examined.Under the optimal conditions,the good linear responses were observed in the concentration range from 0.04～8.37 μg·ml-1,detection limits were 0.017 μg·ml-1.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2011年第12期2851-2853,共3页
Lishizhen Medicine and Materia Medica Research
基金
国家自然科学基金(No.30970219)
吉林省教育厅"十二五"基金项目(No.2011189)
吉林师范大学硕
博启动资金项目(No.2005089)
吉林省四平市科技局项目(No.2009020)
关键词
甘草苷
荧光光谱
Liquiritin
Fluorescence spectrometry
