抗纤灵冲剂对单侧输尿管梗阻大鼠CTGF基因调控的影响

The Impact of Kang XianLing Granule on Gene Expression of CTGF in Unilateral Ureter Obstruction Rats

目的探讨抗纤灵冲剂防治肾间质纤维化可能的作用靶点及分子生物学机制。方法将72只SPF级SD大鼠中54只行经典单侧输尿管结扎,制备UUO大鼠模型,造模成功后分为模型组、抗纤灵组、氯沙坦组。药物干预后分批于14d,21d,28d处死,留取左侧肾组织,运用PCR Array法,检测肾间质纤维化基因CTGF的阳性表达。结果各时间点模型组CTGF基因水平分别较假手术组上调7.12倍、8.26倍及6.55倍,均>2倍,具有非常显著差异。各时间点抗纤灵组CTGF基因表达分别比模型组下调1.79倍、1.63倍、1.63倍,下调均>1.5倍,具有明显差异。结论抗纤灵冲剂作用靶点及分子生物学机制之一是有效诱导CTGF基因表达下调而发挥抗肾间质纤维化效应。

Objective To explore the possible target and molecular biology mechanism of Kang Xianling granule on prevention and cure renal interstitial fibrosis. Methods 72 specified-pathogens free SD rats,in which 54 of them were underwent UUO operation,were randomly divided into sham-operation group,model group,Kang Xianling（KXL） group and Losartan group.After curing for 14,21 and 28 days,we killed them to get nephridial tissues,applied PCR Array to determine the gene expression of CTGF. Results Compared with sham-operation groups,the gene expression of CTGF rose 7.12,8.26 and 6.55 times at model groups in different time points.All of them rose more than 2 times,showing significant difference.On the other hand,the gene expression of CTGF in Kang Xianling（KXL） groups declined 1.79,1.63 and 1.63 times compared with the model groups.All of them decreased more than1.5 times,showing significant difference. Conclusion One of the targets and molecular biology mechanisms of Kang Xianling granule to give play to resist renal interstitial fibrosis is effectively reducing the gene expression of CTGF.