家兔超数排卵的初步试验

A PRELIMINARY STUDY ON THE SUPEROVULATION OF RABBITS

本研究对家兔超排各种处理方法的超排效果进行了比较,并对处理过程中外周血浆 P 及17β～E_2的变化情况进行了放射免疫测定。当 FSH 用于兔的超排时,持续处理三天对于保证 FSH 的超排效果是必需的。FSH 的超排效果显著优于 PMSG(P<0.05),但在注射 PMSG 的同时注射10—12单位 FSH 则可使 PMSG 的超排效果有所提高。受试兔重复处理后,排卵数降低不明显(P>0.05),仅胚胎收集数明显下降(P<0.05),这说明重复处理对受试兔的超排反应的影响不明显,胚眙收集数下降可能是由于手术后的粘连所致。胚胎收集数与处理瞬前及输精瞬前的 P 浓度成负相关(r=-0.3616,-0.4334,P<0.05),与17β—E_2浓度成正相关(r=0.5036,0.5055,P<0.01);且受试兔经 PMSG 处理后的 P 升高幅度远较FSH 处理后为大(P<0.01)。由此说明:PMSG 超排效果较差的原因可能是其 LH的活性较高,引起卵泡的黄体化及带血卵泡的形成所致。

The efficiency of the different treatmental methods for superovulation of rabbits was compared and the concentrations of P and 17β-E_2 in peripheral plasma during treatment were measured by RIA in present study.When FSH was employed in the superovulation of rabbits injection of FSH with a duration of at least 3 days was neccessary to ensure its efficiency in superovulation,The efficiency of FSH in superovulating was significantly superior to that of PMSG,but the efficiency of PMSG could be improved by injection of 10-12 unit FSH at the time PMSG was adminstered.After the rabbits were repeatedly treated,the average number of embryos recovered per danor decreased significantly(P<0.05)but the average ovulation rate did not decreased significantly(P>0.05).It indicats that repeated treatment does not affect the responses of rabbits to superovulating,the decrease of the number of embryos recovered may be due to the adhesion after surgical. The number of embryos recovered per danor was negatively correlated with the levels of P(r=-0.3616,-0.4334,P<0.05)and positively correla ted with the levels of 17-E(r=0.5036,0.5055,P<0.01)both at beginning treatment and insemination.The magnit tude increased of P was significantly higher in rabbits treated with PMSG than in rabbits treated with FSH after treatment.It shows that the poor efficiency of PMSG for superovulation of rabbits may be due to its high activity of LH which can result in luteinization of follicle and formation of blood follicle.