中国甘薯病毒种类的血清学和分子检测

Serological and molecular detection of viruses infecting sweet potato in China

2009～2010年,从我国18个省(市)采集了176份表现病毒病症状的甘薯样品。利用血清学、PCR和核苷酸序列测定的方法,对上述样品中的病毒种类进行了鉴定。血清学检测结果表明,供试样品中甘薯羽状斑驳病毒(SPFMV)的阳性率最高,达56.3%,其次为甘薯G病毒(SPVG)和甘薯类花椰菜花叶病毒(SPCaLV),阳性率分别为34.1%和33.5%。PCR和核苷酸序列测定结果表明,我国甘薯上至少存在SPFMV、SPVG、甘薯潜隐病毒(SPLV)、甘薯褪绿斑病毒(SPCFV)、甘薯褪绿矮化病毒(SPCSV)、黄瓜花叶病毒(CMV)、甘薯脉花叶病毒(SPVMV)和甘薯卷叶病毒(SPLCV)8种病毒。此外,供试样品中没有检测出甘薯轻斑驳病毒(SPMMV),是否存在甘薯轻斑点病毒(SPMSV)、SPCaLV和C-6病毒尚不能确定。

One hundred and seventy-six symptomatic samples were collected from 18 provinces of China in 2009 and 2010, and analysed by using serological, polymerase chain reaction （PCR） and nucleotide sequencing methods. Serological detection results indicated that, Sweet potato feathery mottle virus （SPFMV） was the most commonly detected, being found in 56. 3% of the samples, followed by Sweet potato virus G （SPVG） and Sweet potato caulimo-like virus （ SPCaLV）, in 34.1% and 33.5% of the samples respectively. PCR and nucleotide sequencing showed that at least 8 viruses including SPFMV, SPVG, Sweet potato latent virus （SPLV） , Sweet potato chlorotic fleck virus （SPCFV） , Sweet potato chlorotic stunt virus （ SPCSV）, Cucumber mosaic virus （ CMV ） , Sweet potato vein mosaic virus （SPVMV） and Sweet potato leaf curl virus （ SPLCV）, infected sweet potato in China. Besides, Sweet potato mild mottle virus （SPMMV） was not detected in the samples by serological and RT-PCR methods. It was not sure if there were Sweet potato mild speckling virus （SPMSV）, SPCaLV and C-6 virus in the samples tested.