香石竹斑驳病毒(CarMV)主要基因的分子变异分析

Molecular variation analysis of main genes of Carnation mottle virus(CarMV)

香石竹斑驳病毒(Carnation mottle virus,CarMV)是侵染香石竹的主要病毒之一。本试验从12个香石竹品种中获得CarMV分离物,通过RT-PCR扩增包含p7、p9、CP 3个主要基因的片段,并对扩增产物进行克隆测序。通过序列比对发现CarMV的p7、p9、CP 3个基因有较高的稳定性,p7基因核苷酸序列相似性为98.10%,氨基酸序列相似性为97.81%,其中氨基酸的第11和14位存在显著差异;p9基因核苷酸序列的相似性为98.80%,氨基酸序列相似性为99.13%,氨基酸序列在第4位差异明显;CP基因核酸序列相似性为97.58%,氨基酸的相似性为98.43%,氨基酸序列的第164和331位的变异存在相关性,整个CP变异位点比较分散。证实p7和p9的变异位点主要集中在暴露与寄主互作相关的N端,推测这是导致病毒变异,与寄主互作变异的重要位点。

Carnation mottle virus （CarMV） is one of the important viruses infecting Carnation. In this study, three genes of p7, p9 and CP of CarMV were isolated from twelve different cultivars of Carnation by RT-PCR and their sequences of nucleotides and amino acids were analyzed. The results showed that the p7, p9 and CP genes had higher stability by sequence alignment. The identities of the nucleotide and the amino acid sequence of p7 gene were 98.10% and 97.81% respectively. There were evident variations at the 11th and 14thof amino acid position of p7 gene. While the identities of the nucleotide and amino acid sequence of p9 gene were 98. 80% and 99.13% respectively. There was clear variations at the 4^th amino acid position of p9 gene. However, the identities of the nucleotide and amino acid sequence of CP gene were 97.58% and 98.43% respectively. There was correlative between the 164^th and 331^th amino acid. Those variations of positions of CP were dispersive. The results also showed that the mutations of p7 and p9 were main located at N-terminal parts which exposed to interact with host. Those variations of positions of CarMV might relate to vial variation and interaction between the virus and host.