啤酒花矮化类病毒及其突变体侵染性克隆的构建和生物学活性研究

Construction and biological activity of infectious clones of HSVd(Hop stunt viroid) and its intramolecular recombinant

啤酒花矮化类病毒重组突变体(HSVd D-15)是世界上报道过的仅有的一个啤酒花矮化类病毒(HSVd)分子内重组突变体。为研究其生物学活性,本文分别构建了含有HSVd和HSVd D-15 cDNA加倍串联序列的重组质粒,并对其在指示植株四叶黄瓜上的侵染活性进行了检测。结果表明:仅在接种含有HSVd cDNA多倍串联序列重组质粒的黄瓜上检测到HSVd的侵染,在同样条件下接种了含有HSVd D-15 cDNA两倍串联序列重组质粒的黄瓜上未检测到子代类病毒。HSVdD-15的生物学活性还需通过改进接种方法或将其接种无毒的自然寄主李树苗木来进一步研究。

HSVd D-15 is the only reported HSVd intramolecular recombinant. To examine it＇ s infectivity, recombinant plasmids contained 2-3 units of HSVd or HSVd D-15 cDNAs were constructed and their infectivity were examined on the indicator plant Cucumis sativus L Suyo. As a result, HSVd was detected only from the plants inoculated with multiple units of HSVd cDNAs, but not from the plants inoculated with multiple units of HSVd D-15 cDNAs. The infectivity of HSVd D-15 should be further confirmed using improved inoculation method or by inoculating it onto viroid-free plums. Construction of the multiple units of HSVd D-15 cDNAs provided foundation for the further study on infectivity of HSVd D-15 on its original host plum.