玉米大斑病菌人工接种方法研究

Artificial inoculation methods for Exserohilum turcicum on maize

玉米是世界和我国主要的粮食、饲料和工业原料作物，然而由于玉米大斑病的危害，使玉米产量损失在30％以上，严重威胁玉米生产安全。利用品种的抗病性是防治玉米大斑病最经济有效的方法。国内外研究人员广泛采用人工接种的方法鉴定品种的抗病性，

The efficient and accurate method of inoculating Exserohilum turcicum on maize is most important for studying the corn leaf blight. In this study, four approaches including spore suspension spraying, inocula- tion with grinding infected maize leaves, inoculation liquid inoculum into the leaf whorl and inoculation with inoculated sorghum seeds were compared with natural induction in fields during different growth stages of maize in the greenhouse and field. The results showed that disease incidence of all varieties tested were up to 100% by inoculating with inoculated sorghum seeds during the booting stage （11 to 12 leaf stages）. This method will not only guarantee the enough pathogen inocula but also be easy to use, and could be an effective way for disease resistance identification in field and greenhouse.