何首乌、制何首乌及何首乌发酵炮制品致泻作用与抗氧化活性的比较研究(英文)

Comparison of laxative and antioxidant activities of raw,processed and fermented Polygoni Multiflori Radix

目的:比较何首乌生品、传统炮制品及发酵炮制品的抗氧化活性及致泻副作用的差异, 评价发酵法在何首乌炮制中的应用。方法:利用铁离子还原抗氧化法(FRAP 法)评价何首乌生品与不同炮制品的抗氧化活性。利用小鼠粪便色点法及炭末推进法评价何首乌生品与不同炮制品的泻下副作用。结果:各种炮制方法均可显著降低何首乌的致泻副作用。其中用Rhizopus sp.发酵的何首乌(FB)可显著降低何首乌致稀便的次数及时间, 传统法炮制的何首乌及用 Rhizopus oryzae 发酵的何首乌(FA)可显著减弱小鼠肠道运动。同时, 在 50%和 95%乙醇提取物组, 发酵品 FA 和 FB 的抗氧化活性都强于传统方法炮制的何首乌。经传统方法炮制后何首乌中二苯乙烯苷含量降低, 但发酵炮制品 FA 中二苯乙烯苷的含量无明显变化, 传统方法炮制品及发酵炮制品 FA 中大黄素及大黄素甲醚含量均增加。结论:何首乌的传统炮制法及发酵炮制法在降低何首乌致泻副作用的同时也会不同程度地减弱其抗氧化活性。其中, 利用米根霉 Rhizopus oryzae 发酵得到的何首乌可在降低致泻副作用的同时最大程度地保留何首乌的抗氧化活性, 该发酵法可能成为何首乌炮制方法中新的研究方向。

AIM： To observe the anti-oxidative activity and adverse laxative effect of raw, traditional processed and fermented products of Polygoni Multiflori Radix （PMR）, and furthermore, to evaluate the fermentation method used in the processing procedure of PMR. METHODS： In vitro ferric reducing antioxidant power （FRAP） assay was carried out to evaluate the anti-oxidative activity. Modulation of normal defecation and effect on gastrointestinal motility in mice were carded out to investigate their adverse laxative effect. RESULTS： Fermented PMR induced less severe laxative adverse effect than Polygoni Multiflori Radix Praeparata （PMRP）. PMR fermented with Rhizopus sp. （FB） could modulate the defecation significantly. The gastrointestinal motility was inhibited by PMRP and PMR fermented with Rhizopus oryzae （FA）. FA and FB showed better antioxidant activity than PMRP in 50% and 95% ethanol group. Contents of 2, 3, 5, 4＇-tetrahydroxy-stilbene-2-O-fl-D-glucoside （TSG） were reduced significantly after traditional processing but maintained after fermentation. Emodin and physcion were increased after traditional processing and fermented with Rhizopus oryzae. CONCLUSION： All processing procedure, including fermentation, might reduce its anti-oxidative activity. However, most of the processed products could lessen the adverse effect on gastrointestinal tract compared to PMR. Fermentation with Rhizopus oryzae was considered as a promising processing method of PMR.