RSAP分子标记技术在园艺植物研究中的应用

RSAP Molecular Marker Technique and Its Application of Horticulture Plants

限制性位点扩增多态性(RSAP)是一种基于PCR的新型植物分子标记技术,具有操作简便、结果稳定、效率较高的特点。该技术以植物基因组上广泛分布的限制性位点位为靶标,通过独特的引物设计和PCR扩增程序,无需限制性酶切环节,只要一个简单的PCR,即可产生基于植物限制性位点的多态性标记。阐述了RSAP的原理与流程,对该技术的优势和应用情况做了总结,并对其前景进行了展望。

Restriction site amplified polymorphism（RSAP）,a novel PCR-based plant marker technique,features easy,reliability,and efficient characteristic.Targeted to restriction sites dispersed among plant genomes,RSAP adopted unique primer design and PCR procedure to generate polymorphic markers at restriction sites,without restricted enzyme digesting.This paper describesd the principle and protocol of RSAP,analyses the advantage and the application of RSAP marker technique,and discusses the prospect of this technique.