乳腺癌患者外周血淋巴细胞热休克蛋白70表达水平及其意义

Expression level and clinical significance of heat shock protein 70 in lymphocytes in peripheral blood of patients with breast cancer

目的:探讨乳腺癌患者外周血淋巴细胞中热休克蛋白70(HSP70)表达水平及其临床意义。方法:用Westernblot方法检测45例乳腺癌患者、43例乳腺良性病变患者及43例正常对照组外周血淋巴细胞中HSP70的表达水平。结果:乳腺癌组、乳腺良性病变组外周血淋巴细胞中HSP70表达水平均高于正常对照组,差异有统计学意义(t=2.173,P<0.05);HSP70表达水平在肿瘤直径>2 cm组高于≤2 cm组,差异有统计学意义(t=-2.781,P<0.01);HSP70与乳腺癌患者年龄、组织学分级、临床分期、是否有淋巴结转移及月经状况无关。结论:术前外周血淋巴细胞中HSP70的检测有一定意义,其表达水平有可能成为判断预后的参考指标。

Objective：To explore the expression level and clinical significance of heat shock protein 70（HSP70） in lymphocytes in peripheral blood of patients with breast cancer. Methods：Western blot was used to detect the expression levels of HSP70 in lymphocytes in peripheral blood of 45 patients with breast cancer,43 patients with benign breast diseases,and 43 cases in normal control group. Results：The expression levels of HSP70 in breast cancer group and benign breast diseases group were significantly higher than that in normal control group（t=2.173,P0.05）;the expression level of HSP70 in diameter of tumor2 cm group was significantly higher than that in diameter of tumor≤2 cm group（t=-2.781,P0.01）;there was no correlation between HSP70 and age,histological grades,clinical stages,lymph node metastasis,and menstrual condition. Conclusion：The detection of HSP70 in lymphocytes in peripheral blood before surgery has a certain significance,its expression level may become a reference indication for predicting prognosis.