AB-8大孔吸附树脂纯化双黄连中金银花、连翘提取物工艺

Purification Technology of Extracts from Lonicerae japonicae and Forsythiae suspensa in Shuanghuanglian by AB-8 Macroporous Resin

目的:优化AB-8大孔吸附树脂纯化双黄连中金银花、连翘提取物的工艺。方法:以提取物中总酚、总黄酮的含量为考察指标,从树脂径高比、吸附浓度、吸附流速、洗脱剂浓度等方面对大孔吸附树脂纯化工艺进行优化。结果:最佳纯化工艺为金银花、连翘提取物,加蒸馏水稀释成1.5 g.mL-1(生药量),树脂柱径高比1∶10,最大吸附量与树脂体积比为1∶3.3,水洗3倍树脂体积,4BV 70%乙醇洗脱。结论:AB-8大孔吸附树脂纯化金银花、连翘提取物的最佳工艺稳定可行。

Objective：To optimize purification technology of extracts from L.japonicae and F.suspensa in Shuanghuanglian by AB-8 macroporous resin.Method： The content of total phenolic and total flavonoids were used as indexes.Purification technology was optimized from proportion of diameter-height,adsorption concentration,adsorption speed and eluent concentration,etc.Result： Optimum purification technology was as follows： extracts of L.japonicae and F.suspensa were diluted to concentration was 1.5 g·mL-1by water,ratio of diameter-height 1∶10,proportion of maximum adsorption to resin volume was 1∶3.3,eluted by 3 BV of water,4 BV 70% ethanol as the eluent.Conclusion： This process of purification technology was stable and feasible.