正交试验法优化赤芍总苷闪式提取工艺

Optimization of Smashing Tissue Extraction Process of Total Paeony Glycoside by Orthogonal Test

目的:优化赤芍总苷闪式提取工艺。方法:利用闪式提取器对赤芍总苷进行提取,HPLC测定含量,并以芍药苷和芍药内酯苷的提取量作为主要指标,采用L9(34)正交试验法,考察总苷闪式提取的最佳工艺。结果:闪式提取赤芍总苷的最佳提取工艺为用30倍量的水浸泡6 h,并采用70 V电压提取2 min。结论:闪式提取法可应用于赤芍总苷的提取,该工艺简单,迅速,具有良好的应用前景。

Objective：To study and optimize smashing tissue extraction technology of total paeony glycoside.Method： Total paeony glycoside was extracted by smashing tissue extractor,the content of paeoniflorin and albiflorin were determined by HPLC,and used it as main indexes.Optimum smashing tissue technology was investigated by L9（34） orthogonal test.Result： Optimum smashing tissue extraction conditions were as follows：Radix paeoniae was dipped in 30 times the amount of water for 6 hours and extracted by smashing tissue extraction for 2 minutes with voltage of 70 V.Conclusion： Smashing tissue extraction method could be used to extract of total paeony glycoside,and it was feasible,simple and rapid and had good application prospect.