摘要
目的:建立高效液相色谱法同时测定不同采收期荭草中荭草素、异荭草素含量的方法。方法:色谱柱为AgilentC18(4.6 mm×150 mm,5μm),流动相乙腈-0.1%磷酸水梯度洗脱,检测波长350 nm,流速1.0 mL.min-1。结果:荭草素在0.068 2～0.682μg(r=0.999 7)线性关系良好,平均回收率为99.94%,RSD 1.08%;异荭草素在0.386～3.860μg(r=0.999 3)线性关系良好,平均回收率为99.67%,RSD 1.09%。结论:方法简便、快捷、准确的特点,可用于荭草药材的质量控制。
Objective：To establish an HPLC method for the simultaneous determination of orientin and isocrientin in different harvest times of Polygonum orientale.Method：Orientin and isoorientin were separated on Agilent C18（4.6 mm×150 mm,5μm） column and detected at 350 nm.The mobile phase was acetonitrile-0.3% phosphoric with gradient elution.The flow rate was 1.0 mL·min-1.Result： Orientin and isoorientin were linear within the range of 0.068 2-0.682 μg（r=0.999 7）,0.386-3.860 μg（r=0.999 3）respectively.The average recovery was 99.94%（1.08%）,99.67%（1.09%）.Conclusion： The method is simple,rapid,and it can be used for the quality control of P.orientale.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第2期62-64,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(30873437)
