摘要
目的:为充分利用怀山药资源,避免资源的浪费,研究怀山药各部位总黄酮的供试品制备方法,探讨怀山药各部位的总黄酮含量的变化。方法:采用乙醇超声提取法,正交设计实验优选怀山药中总黄酮的供试品制备方法,分光光度法测定含量。结果:怀山药总黄酮的供试品制备方法为95%乙醇15 mL超声提取3次,每次提取45 min。分光光度法其测定怀山药叶中黄酮含量最高,其次是生山药皮粉、藤茎、山药零余子。结论:怀山药各部位总黄酮含量有显著差异。
Objective:Studying the best extraction method of flavonoids and its change in various parts of Dioscorea opposita Thunb to take full advantage of yam resources and to avoid wastage.Method: Using ethanol ultrasonic extraction method and orthogonal experimental design to select the best extraction method of total flavonoids and determing the content by spectrophotometry.Result: The extraction method of total flavonoids in D.opposita is: ultrasonic extraction with 15 mL of 95% ethyl alcohol 3 times,each time 45 min.The leaf flavonoid content is the highest,followed by skin raw yam flour,cane,yams Bubil.Conclusion: Total flavonoid content in D.opposita in different parts were significantly different.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第2期65-68,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家"十二五"科技重大专项课题(2011BAI06B04)
关键词
怀山药
总黄酮
含量测定
分光光度法
Dioscorea opposita Thunb
total flavonoids
content determination
spectrophotometry