HPLC同时测定益心舒片中五味子醇甲、五味子酯甲、五味子甲素、五味子乙素和丹参酮Ⅱ_A的含量

Determination of Schizandrin,Schisantherin A,Deoxyschizandrin, γ-Schishandrin and Tanshinone Ⅱ_A in Yixinshu Tablet by HPLC

目的:建立HPLC同时测定益心舒片中五味子醇甲、五味子酯甲、五味子甲素、五味子乙素和丹参酮ⅡA的含量。方法:采用Phenomenex Kinetex C18柱,以乙腈-水梯度洗脱,流速1 mL.min-1,柱温30℃,检测波长250 nm。结果:五味子醇甲在21.56～215.6 mg.L-1(r=0.999 9),五味子酯甲在6.500～65.00 mg.L-1(r=1),五味子甲素在3.460～34.60 mg.L-1(r=0.999 9),五味子乙素在6.920～69.20 mg.L-1(r=0.999 9),丹参酮ⅡA在2.400～24.00 mg.L-1(r=0.999 9)呈良好的线性关系,平均回收率五味子醇甲为97.92%,RSD 2.34%(n=6);五味子酯甲为100.28%,RSD 2.57%(n=6);五味子甲素为98.34%,RSD 2.73%(n=6);五味子乙素为98.06%,RSD 1.61%(n=6);丹参酮ⅡA为99.13%,RSD 1.42%(n=6);结论:本法简便、灵敏度高、重复性好,可用于益心舒片的质量控制。

Objective：To establish a HPLC method for the determination of schizandrin,schisantherin A,deoyschizandrin,γ-schishandrin and tanshinone ⅡA in Yixinshu tablet.Method： The Phenomenex Kinetex C18 column was used with a mobile phase of acetonitrile-water by gradient elution,the flow rate was 1 mL·min-1,the column temperature was 30 ℃,the detection wavelength was at 250 nm.Result：The linear ranges of schizandrin,schisantherin A,deoxyschizandrin,γ-schishandrin and tanshinone ⅡA were 21.56-215.6 mg·L-1（r=0.999 9）,6.500-65.00 mg·L-1（r=1）,3.460-34.60 mg·L-1（r=0.999 9）,6.920-69.20 mg·L-1（r=0.999 9） and 2.400-24.00 mg·L-1（r=0.999 9） respectively.The average recoveries of five components were 97.92% with RSD 2.34%,100.28% with RSD 2.57%,98.34% with RSD 2.73%,98.06%with RSD 1.61% and 99.13% with RSD 1.42%.Conclusion：The method is simple,sensitive and repeatable.It can be a method for quality control of Yixinshu tablet.